Single-cell RNA sequencing offers novel perspectives in viral infection research
InfectologiaResumo original em inglês
O que aconteceu
The global emergence of multiple viral zoonoses underscores the substantial threats of viral infections to human health. Given the dynamic and complex mechanisms underlying viral pathogenesis, sophisticated approaches are requisite to advance viral research. Here, we present a systematic review of single-cell RNA sequencing (scRNA-seq), a high-throughput technology enabling transcriptomic profiling at the individual cell level, focusing on its pivotal role in elucidating heterogeneous host cellular responses to viral infection and deciphering underlying pathogenic mechanisms. We summarize scRNA-seq's developmental milestones, compare characteristics of various platforms, and outline its key applications in viral infection research: identifying infection-induced novel cell types/subpopulations, characterizing virus-specific host cell gene expression changes, defining viral target cells, elucidating antiviral immune mechanisms, and clarifying in vivo viral distribution and pathogenesis. By synthesizing these information, this review offers novel research and technical perspectives for dissecting the dynamic and complex virus-host interactions, aiding future advancements in viral infection research.
Resumo publicado sob licença CC BY. A tradução para o português ainda não chegou a este estudo.
O estudo original
Single-cell RNA sequencing offers novel perspectives in viral infection research
Hao Zhang, Yafei Li, Hang Li, Shaomeng Liu, Dang Wang, Huanchun Chen, Qingyun Liu, Xiangru Wang
Descritores MeSH
- Virus Diseases
- Host-Pathogen Interactions
- Single-Cell Analysis
- Viruses
- Sequence Analysis, RNA
- Single-Cell Gene Expression Analysis
- Transcriptome
- Animals
- Humans
- Gene Expression Profiling
Veja também
Estudos relacionados
- Deciphering cell-type-and temporally specific matrisome expression signatures in human cortical development and neurodevelopmental disorders via scRNA-seq meta-analysis
- Research progress on the application of single-cell sequencing in autoimmune diseases
- Integração de GWAS e análise de células únicas para psoríase identifica subtipos celulares associados à doença e alvos terapêuticos
- A systematic review of single-cell RNA sequencing applications and insights in Oryza sativa response to environmental stresses